Short Communication 


Improved method of using scanning electron microscopy to study the morphology of Isodidae family

Santhana Raj Louis, Nur Afrina Muhamad Hendri, Nur Dayana Martin Abdullah, Kamesh Rajendran, Selvanesan Sengol, Mariana Ahamad.

Abstract
Preparation method for scanning electron microscope is a fundamental process in achieving an excellent result in getting ultrastructure micrograph of a house dust mite sample. The objectives of this study are therefore to determine a simpler method of preparing the sample for a better view and excellent images using SEM and to compare the outcome images of its morphological characters based on the new preparation method of which glutaraldehyde and osmium tetra-oxide technique in fixation was replaced with the use of acetone. This modified method gives improved images in SEM and it is very facile as it reduces the processing time by 65 % when compared to the glutaraldehyde and osmium tetra-oxide method.

Key words: House dust mite, scanning electron microscope (SEM), glutaraldehyde, osmium tetra-oxide, acetone, ethanol.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Santhana Raj Louis
Articles by Nur Afrina Muhamad Hendri
Articles by Nur Dayana Martin Abdullah
Articles by Kamesh Rajendran
Articles by Selvanesan Sengol
Articles by Mariana Ahamad
on Google
on Google Scholar
Article Statistics
 Viewed: 1065
Downloaded: 16


How to Cite this Article
Pubmed Style

SRL, Hendri NAM, Abdullah NDM, Rajendran K, Sengol S, Ahamad M. Improved method of using scanning electron microscopy to study the morphology of Isodidae family. AJMS. 2020; 3(1): 1-3. doi:10.5455/ajms.26


Web Style

SRL, Hendri NAM, Abdullah NDM, Rajendran K, Sengol S, Ahamad M. Improved method of using scanning electron microscopy to study the morphology of Isodidae family. http://www.ajms.tk/?mno=60083 [Access: April 19, 2020]. doi:10.5455/ajms.26


AMA (American Medical Association) Style

SRL, Hendri NAM, Abdullah NDM, Rajendran K, Sengol S, Ahamad M. Improved method of using scanning electron microscopy to study the morphology of Isodidae family. AJMS. 2020; 3(1): 1-3. doi:10.5455/ajms.26



Vancouver/ICMJE Style

SRL, Hendri NAM, Abdullah NDM, Rajendran K, Sengol S, Ahamad M. Improved method of using scanning electron microscopy to study the morphology of Isodidae family. AJMS. (2020), [cited April 19, 2020]; 3(1): 1-3. doi:10.5455/ajms.26



Harvard Style

, S. R. L., Hendri, . N. A. M., Abdullah, . N. D. M., Rajendran, . K., Sengol, . S. & Ahamad, . M. (2020) Improved method of using scanning electron microscopy to study the morphology of Isodidae family. AJMS, 3 (1), 1-3. doi:10.5455/ajms.26



Turabian Style

, Santhana Raj Louis, Nur Afrina Muhamad Hendri, Nur Dayana Martin Abdullah, Kamesh Rajendran, Selvanesan Sengol, and Mariana Ahamad. 2020. Improved method of using scanning electron microscopy to study the morphology of Isodidae family. Arabian journal of medical sciences, 3 (1), 1-3. doi:10.5455/ajms.26



Chicago Style

, Santhana Raj Louis, Nur Afrina Muhamad Hendri, Nur Dayana Martin Abdullah, Kamesh Rajendran, Selvanesan Sengol, and Mariana Ahamad. "Improved method of using scanning electron microscopy to study the morphology of Isodidae family." Arabian journal of medical sciences 3 (2020), 1-3. doi:10.5455/ajms.26



MLA (The Modern Language Association) Style

, Santhana Raj Louis, Nur Afrina Muhamad Hendri, Nur Dayana Martin Abdullah, Kamesh Rajendran, Selvanesan Sengol, and Mariana Ahamad. "Improved method of using scanning electron microscopy to study the morphology of Isodidae family." Arabian journal of medical sciences 3.1 (2020), 1-3. Print. doi:10.5455/ajms.26



APA (American Psychological Association) Style

, S. R. L., Hendri, . N. A. M., Abdullah, . N. D. M., Rajendran, . K., Sengol, . S. & Ahamad, . M. (2020) Improved method of using scanning electron microscopy to study the morphology of Isodidae family. Arabian journal of medical sciences, 3 (1), 1-3. doi:10.5455/ajms.26