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Validation of Sterilization of Lucilia cuprina Larvae using Raw Honey through Scanning Electron Microscope

Nur Afrina Muhamad Hendri , Santhana Raj Louis, Nur Fateha Raman, Wan Nur Alia Wan Zahari, Kamesh Rajendran, Nazni Wasi Ahmad.

Abstract
Background: In the application of maggot debridement therapy (MDT), sterilized maggots (Lucilia cuprina) by standard chemicals were used. Recent potential demands who want to use a procedure with all natural products, raw honey has been proposed as a natural sterilization technique to replace the chemical products.

Methods: Lucilia cuprina collected from a laboratory colony were divided into treated group (sterilized) and control group (non-sterilized) to undergo sterility testing with honey dilution. Scanning electron microscope (SEM) was used to examine and ascertain the sterility of honey dilution. SEM provided direct observation of microorganism’s presence on external surface L. cuprina after sterilization by disinfectants of raw honey (tualang honey).

Results: Sixty maggots were examined with 30 in each group under SEM in three magnifications: ×300, ×1500, and ×6500. Treated group showed the absence of microorganism on the surface body segment of the larvae. It reflected in different magnifications in which treated maggots appeared to be smooth and clear of any external contaminants. The untreated maggots exhibited clusters of microorganisms on the external body surface.

Conclusion: This study confirmed the complete sterilization of maggots using raw honey as the antibacterial agent by using SEM due to its disinfection efficacy in destroying microorganisms in L.cuprina.

Key words: Sterilization; maggots; raw honey; scanning electron microscopy.


 
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How to Cite this Article
Pubmed Style

NAMH, Louis SR, Raman NF, Zahari WNAW, Rajendran K, Ahmad NW, . Validation of Sterilization of Lucilia cuprina Larvae using Raw Honey through Scanning Electron Microscope. AJMS. 2020; 3(1): 11-14. doi:10.5455/ajms.29


Web Style

NAMH, Louis SR, Raman NF, Zahari WNAW, Rajendran K, Ahmad NW, . Validation of Sterilization of Lucilia cuprina Larvae using Raw Honey through Scanning Electron Microscope. http://www.ajms.tk/?mno=59533 [Access: April 18, 2020]. doi:10.5455/ajms.29


AMA (American Medical Association) Style

NAMH, Louis SR, Raman NF, Zahari WNAW, Rajendran K, Ahmad NW, . Validation of Sterilization of Lucilia cuprina Larvae using Raw Honey through Scanning Electron Microscope. AJMS. 2020; 3(1): 11-14. doi:10.5455/ajms.29



Vancouver/ICMJE Style

NAMH, Louis SR, Raman NF, Zahari WNAW, Rajendran K, Ahmad NW, . Validation of Sterilization of Lucilia cuprina Larvae using Raw Honey through Scanning Electron Microscope. AJMS. (2020), [cited April 18, 2020]; 3(1): 11-14. doi:10.5455/ajms.29



Harvard Style

, N. A. M. H. ., Louis, S. R., Raman, N. F., Zahari, W. N. A. W., Rajendran, K., Ahmad, N. W. & (2020) Validation of Sterilization of Lucilia cuprina Larvae using Raw Honey through Scanning Electron Microscope. AJMS, 3 (1), 11-14. doi:10.5455/ajms.29



Turabian Style

, Nur Afrina Muhamad Hendri , Santhana Raj Louis, Nur Fateha Raman, Wan Nur Alia Wan Zahari, Kamesh Rajendran, Nazni Wasi Ahmad, and . 2020. Validation of Sterilization of Lucilia cuprina Larvae using Raw Honey through Scanning Electron Microscope. Arabian journal of medical sciences, 3 (1), 11-14. doi:10.5455/ajms.29



Chicago Style

, Nur Afrina Muhamad Hendri , Santhana Raj Louis, Nur Fateha Raman, Wan Nur Alia Wan Zahari, Kamesh Rajendran, Nazni Wasi Ahmad, and . "Validation of Sterilization of Lucilia cuprina Larvae using Raw Honey through Scanning Electron Microscope." Arabian journal of medical sciences 3 (2020), 11-14. doi:10.5455/ajms.29



MLA (The Modern Language Association) Style

, Nur Afrina Muhamad Hendri , Santhana Raj Louis, Nur Fateha Raman, Wan Nur Alia Wan Zahari, Kamesh Rajendran, Nazni Wasi Ahmad, and . "Validation of Sterilization of Lucilia cuprina Larvae using Raw Honey through Scanning Electron Microscope." Arabian journal of medical sciences 3.1 (2020), 11-14. Print. doi:10.5455/ajms.29



APA (American Psychological Association) Style

, N. A. M. H. ., Louis, S. R., Raman, N. F., Zahari, W. N. A. W., Rajendran, K., Ahmad, N. W. & (2020) Validation of Sterilization of Lucilia cuprina Larvae using Raw Honey through Scanning Electron Microscope. Arabian journal of medical sciences, 3 (1), 11-14. doi:10.5455/ajms.29