Original Research (Original Article) 


Differential microsatellites patterns in Nile tilapia following Aeromonas hydrophila infection

Ghada A Awadalla, Mohammed A. El-Magd, Azza E Hassan, lamiaa A Barakat, Gehad R Elsayed.

Abstract
Aim: This study was performed to compare between microsatellites patterns in Nile tilapia following infection with Aeromonas hydrophila. Materials and methods: UNH102, UNH106, UNH115, and UNH123 microsatellites were amplified by PCR using genomic DNA extracted from whole blood samples of control (unchallenged), resistant and diseased (challenged by A. hydrophila) fish. These four microsatellite markers were amplified from 10 fish in each group and separated on a 4% agarose gel electrophoresis. Results: Analysis of these microsatellite polymorphic markers in both resistant and diseased fish showed heterozygosity (different band numbers). Conclusion: These data infer the presence of variations in microsatellites patterns between resistant and susceptible Nile tilapia to A. hydrophila infection. Therefore, microsatellites could be applied as useful markers for selection of fish with higher immune repose against bacterial infection.

Key words: Microsatellites; Nile tilapia; Aeromonas hydrophila; PCR


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Ghada A Awadalla
Articles by Mohammed A. El-Magd
Articles by Azza E Hassan
Articles by lamiaa A Barakat
Articles by Gehad R Elsayed
on Google
on Google Scholar
Article Statistics
 Viewed: 127
Downloaded: 14
Cited: 0


How to Cite this Article
Pubmed Style

Awadalla GA, El-Magd MA, Hassan AE, Barakat lA, Elsayed GR. Differential microsatellites patterns in Nile tilapia following Aeromonas hydrophila infection. AJMS. 2019; 2(2): 51-53. doi:10.5455/ajms.25


Web Style

Awadalla GA, El-Magd MA, Hassan AE, Barakat lA, Elsayed GR. Differential microsatellites patterns in Nile tilapia following Aeromonas hydrophila infection. http://www.ajms.tk/?mno=54800 [Access: July 19, 2019]. doi:10.5455/ajms.25


AMA (American Medical Association) Style

Awadalla GA, El-Magd MA, Hassan AE, Barakat lA, Elsayed GR. Differential microsatellites patterns in Nile tilapia following Aeromonas hydrophila infection. AJMS. 2019; 2(2): 51-53. doi:10.5455/ajms.25



Vancouver/ICMJE Style

Awadalla GA, El-Magd MA, Hassan AE, Barakat lA, Elsayed GR. Differential microsatellites patterns in Nile tilapia following Aeromonas hydrophila infection. AJMS. (2019), [cited July 19, 2019]; 2(2): 51-53. doi:10.5455/ajms.25



Harvard Style

Awadalla, G. A., El-Magd, . M. A., Hassan, . A. E., Barakat, . l. A. & Elsayed, . G. R. (2019) Differential microsatellites patterns in Nile tilapia following Aeromonas hydrophila infection. AJMS, 2 (2), 51-53. doi:10.5455/ajms.25



Turabian Style

Awadalla, Ghada A, Mohammed A. El-Magd, Azza E Hassan, lamiaa A Barakat, and Gehad R Elsayed. 2019. Differential microsatellites patterns in Nile tilapia following Aeromonas hydrophila infection. Arabian journal of medical sciences, 2 (2), 51-53. doi:10.5455/ajms.25



Chicago Style

Awadalla, Ghada A, Mohammed A. El-Magd, Azza E Hassan, lamiaa A Barakat, and Gehad R Elsayed. "Differential microsatellites patterns in Nile tilapia following Aeromonas hydrophila infection." Arabian journal of medical sciences 2 (2019), 51-53. doi:10.5455/ajms.25



MLA (The Modern Language Association) Style

Awadalla, Ghada A, Mohammed A. El-Magd, Azza E Hassan, lamiaa A Barakat, and Gehad R Elsayed. "Differential microsatellites patterns in Nile tilapia following Aeromonas hydrophila infection." Arabian journal of medical sciences 2.2 (2019), 51-53. Print. doi:10.5455/ajms.25



APA (American Psychological Association) Style

Awadalla, G. A., El-Magd, . M. A., Hassan, . A. E., Barakat, . l. A. & Elsayed, . G. R. (2019) Differential microsatellites patterns in Nile tilapia following Aeromonas hydrophila infection. Arabian journal of medical sciences, 2 (2), 51-53. doi:10.5455/ajms.25