Original Research (Original Article) 


Chitosan nanoparticles from Artemia salina attenuate LPS-induced inflammation in MCF7 cells

Abeer A. Khamis, Mai M. Elkeiy, Mohammed A. El-Magd, Mona M. El Gamal.

Abstract
The present study was carried out to investigate the influence of Artemia salina-derived chitosan nanoparticles (CNPs) in lipopolysaccharide (LPS)-induced inflammation in the human breast cancer MCF7 cells. The in vitro inhibition assays exhibited a weak inhibitory activity for CNPs against COX1 isozyme and a strong inhibitory activity against COX2 isozyme. Treatment the cells with CNPs decreased viability of MCF7. Administration of CNPs also induced an anti-inflammatory effect in LPS-induced inflamed MCF7 cells as indicated by results obtained from real time PCR which showed a significant downregulated expression of the inflammatory cytokines NFkB, IL6, COX1, and COX2 mRNA. In all experiments, CNPs exerted more potent cytotoxic effect on MCF7 and higher anti-inflammatory effect on inflamed MCF7 than chitosan. These data conclude that CNPs could be used as a promise anti-inflammatory agent.

Key words: Chitosan nanoparticles; Artemia salina; MCF7; Inflammation


 
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How to Cite this Article
Pubmed Style

Khamis AA, Elkeiy MM, El-Magd MA, Gamal MME. Chitosan nanoparticles from Artemia salina attenuate LPS-induced inflammation in MCF7 cells. AJMS. 2019; 2(2): 44-47. doi:10.5455/ajms.23


Web Style

Khamis AA, Elkeiy MM, El-Magd MA, Gamal MME. Chitosan nanoparticles from Artemia salina attenuate LPS-induced inflammation in MCF7 cells. http://www.ajms.tk/?mno=54358 [Access: November 18, 2019]. doi:10.5455/ajms.23


AMA (American Medical Association) Style

Khamis AA, Elkeiy MM, El-Magd MA, Gamal MME. Chitosan nanoparticles from Artemia salina attenuate LPS-induced inflammation in MCF7 cells. AJMS. 2019; 2(2): 44-47. doi:10.5455/ajms.23



Vancouver/ICMJE Style

Khamis AA, Elkeiy MM, El-Magd MA, Gamal MME. Chitosan nanoparticles from Artemia salina attenuate LPS-induced inflammation in MCF7 cells. AJMS. (2019), [cited November 18, 2019]; 2(2): 44-47. doi:10.5455/ajms.23



Harvard Style

Khamis, A. A., Elkeiy, . M. M., El-Magd, . M. A. & Gamal, . M. M. E. (2019) Chitosan nanoparticles from Artemia salina attenuate LPS-induced inflammation in MCF7 cells. AJMS, 2 (2), 44-47. doi:10.5455/ajms.23



Turabian Style

Khamis, Abeer A., Mai M. Elkeiy, Mohammed A. El-Magd, and Mona M. El Gamal. 2019. Chitosan nanoparticles from Artemia salina attenuate LPS-induced inflammation in MCF7 cells. Arabian journal of medical sciences, 2 (2), 44-47. doi:10.5455/ajms.23



Chicago Style

Khamis, Abeer A., Mai M. Elkeiy, Mohammed A. El-Magd, and Mona M. El Gamal. "Chitosan nanoparticles from Artemia salina attenuate LPS-induced inflammation in MCF7 cells." Arabian journal of medical sciences 2 (2019), 44-47. doi:10.5455/ajms.23



MLA (The Modern Language Association) Style

Khamis, Abeer A., Mai M. Elkeiy, Mohammed A. El-Magd, and Mona M. El Gamal. "Chitosan nanoparticles from Artemia salina attenuate LPS-induced inflammation in MCF7 cells." Arabian journal of medical sciences 2.2 (2019), 44-47. Print. doi:10.5455/ajms.23



APA (American Psychological Association) Style

Khamis, A. A., Elkeiy, . M. M., El-Magd, . M. A. & Gamal, . M. M. E. (2019) Chitosan nanoparticles from Artemia salina attenuate LPS-induced inflammation in MCF7 cells. Arabian journal of medical sciences, 2 (2), 44-47. doi:10.5455/ajms.23