Original Research (Original Article) 


The potential ameliorative effects of morin and rutin against titanium dioxide nanoparticles-induced hepatotoxicity in rats

Reham Eid, Mohamed Sherif, Al-Shimaa Abas, Mohamed Hussein.

Abstract
Background: Titanium dioxide nanoparticles (TiO2 NPs) are well known as a photocatalyst and it is widely used for photocatalysed decomposition, sensor elements, water purification and have many other application, however they also have toxic effect on animals exposed to them. Objectives: we investigated the potential ameliorative effect for the two flavonoids morin and rutin which have apotent antioxidant activity, against TiO2 nanoparticles-induced toxicities in rat liver. Materials and methods: Sprague-dawly rats (n =70, weight 150-200g) were divided into seven groups. Group (G1) (control), G2 (treated with morin 30 mg/kg), G3 (treated with rutin 80 mg/kg), G4 (toxic group that fed with titanium dioxide nanoparticles 100 mg/kg), G5 (TiO2 NPs+ morin), G6 (TiO2 NPs+ rutin), G7 (TiO2 NPs+ morin+ rutin). The evaluated parameters included liver function markers, some minerals and electrolytes, antioxidant (superoxide dismutase SOD, catalase CAT and glutathione GSH) and oxidative stress malondialdehyde (MDA) biomarkers in addition to DNA fragmentation and histopathological examination of liver tissues. Results: These experimental studies showed that a significant increase of Alanine aminotranseferase (ALT),Aspartate aminotranseferase (AST), Alkaline Phosphatase (ALP) activities in liver functions, potassium (K), phosphorous (Ph) levels in minerals, MDA content, comet, tail length, DNA in tail and tail moment of DNA fragmentation were detected in TiO2 NPs (G4) administrated group indicating liver damage which compared with control group (G1) but decrease significantly in this parameters when used morin or rutin in (G5) or (G6) which compared with toxic group (G4), so (G7) combination groups succeeded to decrease significantly in this parameters and returns to (G1). Another experimental studies showed that a significant decrease in total protein, albumin, sodium (Na), calcium (Ca) levels of minerals, glutathione (GSH) level, superoxide dismutase (SOD) and catalase (CAT) activities in toxic groups (G4) but we succeeded to increase significantly in this parameters in G5, G6 and G7 that compared with control group and restore it. Conclusion: TiO2NPs oral administration induced toxic effects and DNA damage in the liver that may be attributable to oxidative stress and administration of morin and/or rutin with TiO2NPs offers protection against their damaging effect.

Key words: Titanium dioxide nanoparticles; Morin; Rutin; Antioxidant.


 
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How to Cite this Article
Pubmed Style

Eid R, Sherif M, Abas A, Hussein M, . The potential ameliorative effects of morin and rutin against titanium dioxide nanoparticles-induced hepatotoxicity in rats. AJMS. 2018; 1(2): 25-30. doi:10.5455/ajms.12


Web Style

Eid R, Sherif M, Abas A, Hussein M, . The potential ameliorative effects of morin and rutin against titanium dioxide nanoparticles-induced hepatotoxicity in rats. http://www.ajms.tk/?mno=14454 [Access: November 18, 2019]. doi:10.5455/ajms.12


AMA (American Medical Association) Style

Eid R, Sherif M, Abas A, Hussein M, . The potential ameliorative effects of morin and rutin against titanium dioxide nanoparticles-induced hepatotoxicity in rats. AJMS. 2018; 1(2): 25-30. doi:10.5455/ajms.12



Vancouver/ICMJE Style

Eid R, Sherif M, Abas A, Hussein M, . The potential ameliorative effects of morin and rutin against titanium dioxide nanoparticles-induced hepatotoxicity in rats. AJMS. (2018), [cited November 18, 2019]; 1(2): 25-30. doi:10.5455/ajms.12



Harvard Style

Eid, R. . ., Sherif, M. . ., Abas, A. . ., Hussein, M. . . & (2018) The potential ameliorative effects of morin and rutin against titanium dioxide nanoparticles-induced hepatotoxicity in rats. AJMS, 1 (2), 25-30. doi:10.5455/ajms.12



Turabian Style

Eid, Reham , Mohamed Sherif, Al-Shimaa Abas, Mohamed Hussein, and . 2018. The potential ameliorative effects of morin and rutin against titanium dioxide nanoparticles-induced hepatotoxicity in rats. Arabian journal of medical sciences, 1 (2), 25-30. doi:10.5455/ajms.12



Chicago Style

Eid, Reham , Mohamed Sherif, Al-Shimaa Abas, Mohamed Hussein, and . "The potential ameliorative effects of morin and rutin against titanium dioxide nanoparticles-induced hepatotoxicity in rats." Arabian journal of medical sciences 1 (2018), 25-30. doi:10.5455/ajms.12



MLA (The Modern Language Association) Style

Eid, Reham , Mohamed Sherif, Al-Shimaa Abas, Mohamed Hussein, and . "The potential ameliorative effects of morin and rutin against titanium dioxide nanoparticles-induced hepatotoxicity in rats." Arabian journal of medical sciences 1.2 (2018), 25-30. Print. doi:10.5455/ajms.12



APA (American Psychological Association) Style

Eid, R. . ., Sherif, M. . ., Abas, A. . ., Hussein, M. . . & (2018) The potential ameliorative effects of morin and rutin against titanium dioxide nanoparticles-induced hepatotoxicity in rats. Arabian journal of medical sciences, 1 (2), 25-30. doi:10.5455/ajms.12