Original Research (Original Article) 


Isolation and characterization of transferrin gene from Nile tilapia

Afaf Abdel-Magid, Fatma Abd Elrazik, Mohamed Agour, Mohammed El-Magd.

Abstract
Background: Transferrin (TF) is the major iron ion transport protein in animals and plants. Pathogenic bacteria have developed a set of mechanisms to obtain iron from the host, even from TF. However, due to its high degree of genetic polymorphisms, TF may be restricted to certain genotypes. Aim: The aim of the present study was to isolate TF gene from Nile tilapia infected with Aeromonas hydrophila and to compare between genotypes and alleles frequencies of infected (diseased) and non-infected (control) fish. Materials and methods: PCR was used to isolate TF gene from blood of control and diseased (challenged by A. hydrophila) fish, then single strand conformational polymorphisms (SSCP) technique was used to genotype these fish, and finally genotypic and allelic frequencies as well as Chi-square (χ2) were calculated in the two fish groups. Results: A partial amplicon of the TF gene (771 bp) was obtained by PCR. Moreover, three different SSCP banding patterns (designed as TT, TC, and CC based on their pattern rather than their nucleotide sequencing) were detected in the examined fishes. The frequencies of TT, TC, and CC genotypes were significantly different between the diseased and the control fish. The frequency of TT was higher in the control fish than CC, however in the diseased fish, CC genotype was higher than TT. Consequently, the frequency of C allele was significantly higher in the diseased fish than the control fish. Additionally, the three genotypes were in Hardy–Weinberg disequilibrium (HWE) because χ2 value was 0.29 (P > 0.05). Conclusion: These data suggest that C may be the risk (recessive or mutant) allele for susceptibility to A. hydrophila infection in Nile tilapia. By comparing the genotypes of the TF gene in diseased and control fish, brood stock having only the resistant alleles can be selected, but first gene sequencing should be applied to determine the type and location of these prospective SNPs.

Key words: Transferrin; Genotype; Nile tilapia; SSCP-PCR


 
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How to Cite this Article
Pubmed Style

Abdel-Magid A, Elrazik FA, Agour M, El-Magd M, . Isolation and characterization of transferrin gene from Nile tilapia. AJMS. 2018; 1(2): 17-19. doi:10.5455/ajms.10


Web Style

Abdel-Magid A, Elrazik FA, Agour M, El-Magd M, . Isolation and characterization of transferrin gene from Nile tilapia. http://www.ajms.tk/?mno=11527 [Access: November 18, 2019]. doi:10.5455/ajms.10


AMA (American Medical Association) Style

Abdel-Magid A, Elrazik FA, Agour M, El-Magd M, . Isolation and characterization of transferrin gene from Nile tilapia. AJMS. 2018; 1(2): 17-19. doi:10.5455/ajms.10



Vancouver/ICMJE Style

Abdel-Magid A, Elrazik FA, Agour M, El-Magd M, . Isolation and characterization of transferrin gene from Nile tilapia. AJMS. (2018), [cited November 18, 2019]; 1(2): 17-19. doi:10.5455/ajms.10



Harvard Style

Abdel-Magid, A., Elrazik, . F. A., Agour, . M., El-Magd, . M. & (2018) Isolation and characterization of transferrin gene from Nile tilapia. AJMS, 1 (2), 17-19. doi:10.5455/ajms.10



Turabian Style

Abdel-Magid, Afaf, Fatma Abd Elrazik, Mohamed Agour, Mohammed El-Magd, and . 2018. Isolation and characterization of transferrin gene from Nile tilapia. Arabian journal of medical sciences, 1 (2), 17-19. doi:10.5455/ajms.10



Chicago Style

Abdel-Magid, Afaf, Fatma Abd Elrazik, Mohamed Agour, Mohammed El-Magd, and . "Isolation and characterization of transferrin gene from Nile tilapia." Arabian journal of medical sciences 1 (2018), 17-19. doi:10.5455/ajms.10



MLA (The Modern Language Association) Style

Abdel-Magid, Afaf, Fatma Abd Elrazik, Mohamed Agour, Mohammed El-Magd, and . "Isolation and characterization of transferrin gene from Nile tilapia." Arabian journal of medical sciences 1.2 (2018), 17-19. Print. doi:10.5455/ajms.10



APA (American Psychological Association) Style

Abdel-Magid, A., Elrazik, . F. A., Agour, . M., El-Magd, . M. & (2018) Isolation and characterization of transferrin gene from Nile tilapia. Arabian journal of medical sciences, 1 (2), 17-19. doi:10.5455/ajms.10