Original Research (Original Article) 


Determination of Etidronate in Pharmaceutical Formulations by RP-HPLC Method with Indirect UV Detection

Mokhtar Mabrouk, Sherin Hammad, Mohamed Abdelaziz, Fotouh Rashed Mansour.

Abstract
A reversed-phase HPLC method with indirect UV detection has been developed for the determination of the non-chromophoric bisphosphonate drug, etidronate disodium, in bulk and in tablet dosage forms. The method is based on adding sodium salicylate as a probe component to the mobile phase. Analyte detection was achieved by monitoring the decrease in the eluent background signal at 210 nm. The method was validated according to the guidelines of the International Conference on Harmoinzation and showed adequate levels of accuracy, precision and specificity for routine quality control purposes. The linearity of the method was studied over the concentration range of 50-300 μg/mL. Calibration plots of the area of negative analyte signals plotted against the concentration showed a high value of the correlation coefficient (0.999), indicating a good linearity of the proposed method. The method was also successfully applied for the analysis of two other non-chromophoric bisphosphonates; alendronate sodium trihydrate and clodronate disodium tetrahydrate. These results prove that the method is applicable for determination of non-chromophoric bisphosphonates.

Key words: Etidronate; Bisphosphonates; Non-chromophoric drugs; Pharmaceutical Tablets; RP-HPLC; Indirect UV Detection


 
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How to Cite this Article
Pubmed Style

Mabrouk M, Hammad S, Abdelaziz M, Mansour FR, . Determination of Etidronate in Pharmaceutical Formulations by RP-HPLC Method with Indirect UV Detection. AJMS. 2018; 1(2): 1-4. doi:10.5455/ajms.7


Web Style

Mabrouk M, Hammad S, Abdelaziz M, Mansour FR, . Determination of Etidronate in Pharmaceutical Formulations by RP-HPLC Method with Indirect UV Detection. http://www.ajms.tk/?mno=10038 [Access: July 19, 2019]. doi:10.5455/ajms.7


AMA (American Medical Association) Style

Mabrouk M, Hammad S, Abdelaziz M, Mansour FR, . Determination of Etidronate in Pharmaceutical Formulations by RP-HPLC Method with Indirect UV Detection. AJMS. 2018; 1(2): 1-4. doi:10.5455/ajms.7



Vancouver/ICMJE Style

Mabrouk M, Hammad S, Abdelaziz M, Mansour FR, . Determination of Etidronate in Pharmaceutical Formulations by RP-HPLC Method with Indirect UV Detection. AJMS. (2018), [cited July 19, 2019]; 1(2): 1-4. doi:10.5455/ajms.7



Harvard Style

Mabrouk, M., Hammad, . S., Abdelaziz, . M., Mansour, . F. R. & (2018) Determination of Etidronate in Pharmaceutical Formulations by RP-HPLC Method with Indirect UV Detection. AJMS, 1 (2), 1-4. doi:10.5455/ajms.7



Turabian Style

Mabrouk, Mokhtar, Sherin Hammad, Mohamed Abdelaziz, Fotouh Rashed Mansour, and . 2018. Determination of Etidronate in Pharmaceutical Formulations by RP-HPLC Method with Indirect UV Detection. Arabian journal of medical sciences, 1 (2), 1-4. doi:10.5455/ajms.7



Chicago Style

Mabrouk, Mokhtar, Sherin Hammad, Mohamed Abdelaziz, Fotouh Rashed Mansour, and . "Determination of Etidronate in Pharmaceutical Formulations by RP-HPLC Method with Indirect UV Detection." Arabian journal of medical sciences 1 (2018), 1-4. doi:10.5455/ajms.7



MLA (The Modern Language Association) Style

Mabrouk, Mokhtar, Sherin Hammad, Mohamed Abdelaziz, Fotouh Rashed Mansour, and . "Determination of Etidronate in Pharmaceutical Formulations by RP-HPLC Method with Indirect UV Detection." Arabian journal of medical sciences 1.2 (2018), 1-4. Print. doi:10.5455/ajms.7



APA (American Psychological Association) Style

Mabrouk, M., Hammad, . S., Abdelaziz, . M., Mansour, . F. R. & (2018) Determination of Etidronate in Pharmaceutical Formulations by RP-HPLC Method with Indirect UV Detection. Arabian journal of medical sciences, 1 (2), 1-4. doi:10.5455/ajms.7